Immunotherapy of Hepatocellular Carcinoma with Magnetic PD-1 Peptide-Imprinted Polymer Nanocomposite and Natural Killer Cells

Biomolecules

Jyun-Ren Chen¹  Hung-Yin Lin¹  Kai-Hsi Liu²  Mei-Hwa Lee³  JamesL. Thomas⁴ 

[1] Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan;Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, Taiwan;Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan;Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA;

关键词: immunotherapy;    human hepatoma cells;    programmed cell death protein 1 (pd-1);    magnetic nanoparticles;    peptide-imprinted polymer;    natural killer cells;   

DOI  :  10.3390/biom9110651

来源: DOAJ

【 摘 要 】

Programmed cell death protein 1 (PD-1) is a biomarker on the surface of cells with a role in promoting self-tolerance by suppressing the inflammatory activity of T cells. In this work, one peptide of PD-1 was used as the template for molecular imprinting to form magnetic peptide-imprinted poly(ethylene-co-vinyl alcohol) composite nanoparticles (MPIP NPs). The nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), Brunauer−Emmett−Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Natural killer 92 (NK-92) cells were added to these composite nanoparticles and then incubated with human hepatoma (HepG2) cells. The viability and the apoptosis pathway of HepG2 were then studied using cell counting kit-8 (CCK8) and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. These nanoparticles were found to significantly enhance the activity of natural killer cells toward HepG2 cells by increasing the expression of nuclear factor kappa B (NF-κB), caspase 8, and especially caspase 3.

【 授权许可】

Unknown