Synthesis of iron-oxide magnetic nanoparticles coated with dextran of varied molecular mass using a facile ball-milling method

Micro & nano letters

article

Bo-Wei Chen¹  Shadie Hatamie²  Prabir Garu¹  Pooyan Heravi³  Jiann-Yeu Chen⁴  Bo-Tau Liu⁵  Zung-Hang Wei³  Da-Jeng Yao¹ 

[1] Institute of NanoEngineering and MicroSystems, National Tsing Hua University;Department of Ophthalmology, National Taiwan University Hospital, College of Medicine, National Taiwan University;Department of Power Mechanical Engineering, National Tsing Hua University;Center of Nanoscience and Nanotechnology, National Chung Hsing University;Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology

关键词: superparamagnetism;    toxicology;    nanoparticles;    cellular biophysics;    nanofabrication;    magnetic particles;    nanomedicine;    nanomagnetics;    cancer;    biomedical materials;    transmission electron microscopy;    precipitation (physical chemistry);    iron compounds;    light scattering;    Raman spectra;    X-ray diffraction;    Fourier transform infrared spectra;    saturation magnetisation;    thermal analysis;    ball milling;    nanocomposites;    co-precipitated superparamagnetic iron oxide nanoparticles;    biomedical applications;    simple ball-milling method;    dextran-coated superparamagnetic iron oxide nanoparticles;    coating dextran;    co-precipitation methods;    saturation magnetisation;    transmission electron microscopy;    superconducting-quantum-interference-device magnetometer;    dynamic light scattering devices;    surface coating percentage;    surface coating;    conventional co-precipitation protocols;    3-(4;    5-dimethylthiazol-2-yl)-2;    5-diphenyltetrazolium bromide assay;    ball-milled DSPION;    dextran-coated SPION;    attenuated-total-reflection Fourier-transform infrared spectra;    molecular mass;    hydrodynamic diameters;    thermogravimetric analysis;    cytotoxicity;    human hepatoma cells;    Prussian blue staining;    Fe2O3;   

DOI  :  10.1049/mnl.2019.0811

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

A simple ball-milling method was implemented to prepare dextran-coated superparamagnetic iron oxide nanoparticles (dextran-coated SPIONs, DSPIONs) by coating dextran (molecular mass, M dextran , 10, 40, 70 and 100 kDa) onto nanoparticles, which synthesised with co-precipitation methods. The size, saturation magnetisation and hydrodynamic diameters of the DSPIONs, composed of Fe 3 O 4 and γ-Fe 2 O 3 proved by X-ray diffraction and Raman spectra, were 11–14 nm, 55–65 emu g −1 and 200–300 nm using transmission electron microscope, superconducting-quantum-interference-device magnetometer and dynamic light scattering devices, respectively. The characteristic lines of surface coating were revealed in attenuated-total-reflection Fourier-transform infrared spectra. The saturation magnetisation and surface coating percentage (thermogravimetric analysis) of the ball-milled DSPIONs were independent on M dextran . The results were different from the statistical data from previous studies: DSPIONs using increasing M dextran were accompanied by decreasing saturation magnetisation and increasing surface coating in conventional co-precipitation protocols. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated the ball-milled DSPIONs exhibited low cytotoxicity in human hepatoma (SK-HEP1) cells, and the implementation of Prussian blue staining proved the DSPIONs were able to be uptaken by SK-HEP1. There was no obvious difference between the ball-milled DSPIONs with various M dextran . It demonstrated the ball milling process might be potential for SPION preparation in the biomedical field.

【 授权许可】

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