Colorimetric biosensors for point-of-care virus detections

Materials Science for Energy Technologies

Xiaodong Zhou¹  Victoria Xin Ting Zhao²  Ten It Wong³  Xin Ting Zheng³  Yen Nee Tan³ 

[1] Faculty of Science, Agriculture &College of Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore;Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 2 Fusionopolis Way, 08-03, Innovis, Singapore 138634, Singapore;

关键词: Colorimetric detection;    Virus;    Loop-mediated isothermal amplification (LAMP);    Bioassay;    Nanoparticle-based biosensors;   

DOI  :  

来源: DOAJ

【 摘 要 】

Colorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.

【 授权许可】

Unknown