An evaluation of a liquid antimicrobial (Sal CURB®) for reducing the risk of porcine epidemic diarrhea virus infection of naïve pigs during consumption of contaminated feed

BMC Veterinary Research

Eric Nelson¹  Jane Christopher-Hennings¹  Travis Clement¹  Casey Neill²  Scott Dee² 

[1] Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, SD, USA;Pipestone Applied Research, Pipestone Veterinary Services, Pipestone 56164, MN, USA

关键词: Formaldehyde;    Bioassay;    Feed;    Sal CURB®;    PEDV;    Virus;    Diarrhea;    Epidemic;    Porcine;   

Others  :  1118962
DOI  :  10.1186/s12917-014-0220-9

 received in 2014-07-02, accepted in 2014-09-18,  发布年份 2014

【 摘 要 】

Background

Since its initial detection in May 2013, porcine epidemic diarrhea virus (PEDV) has spread rapidly throughout the US swine industry. Recently, contaminated feed was confirmed as a vehicle for PEDV infection of naïve piglets. This research provides in vivo data supporting the ability of a liquid antimicrobial product to reduce this risk.

Results

Sal CURB® (Kemin Industries, Des Moines, IA, USA) is a FDA-approved liquid antimicrobial used to control Salmonella contamination in poultry and swine diets. To test its effect against PEDV, Sal CURB®-treated feed was spiked with a stock isolate of PEDV (Ct = 25.22), which PEDV-naïve piglets were allowed to ingest via natural feeding behavior (ad libitum) for a 14-day period. For the purpose of a positive control, a separate group of piglets was allowed to ingest non-treated (Sal CURB®-free) feed also spiked with stock PEDV (Ct = 25.22). A negative control group received PEDV-free feed. Clinical signs of PEDV infection (vomiting and diarrhea) and viral shedding in feces were observed in the positive control group 2–3 days post-consumption of non-treated feed. In contrast, no evidence of infection was observed in pigs fed Sal CURB®-treated feed or in the negative controls throughout the 14-day study period. In addition, the Sal CURB®-treated feed samples had higher (p < 0.0001) mean PEDV Ct values than samples from the positive control group.

Conclusions

These data provide proof of concept that feed treated with Sal CURB® can serve as a means to reduce the risk of PEDV infection through contaminated feed. Furthermore, the results from the positive control group provide additional proof of concept regarding the ability of contaminated feed to serve as a risk factor for PEDV infection of naïve piglets.

【 授权许可】

   
2014 Dee et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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