Allogeneic Embryos Disregulate Leukemia Inhibitory Factor (LIF) and Its Receptor in the Porcine Endometrium During Implantation

Frontiers in Veterinary Science

Inmaculada Parrilla²  Amaia Jauregi-Miguel²  Emilio A. Martinez²  Manuel Alvarez-Rodriguez³  Maria A. Gil³  Josep M. Cambra³  Heriberto Rodriguez-Martinez⁴  Cristina A. Martinez⁵  Cristina Cuello⁵ 

[1] Clinical Sciences (BKV), BKH/Obstetrics &Gynaecology, Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden;;Department of Biomedical &Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research “Campus Mare Nostrum”, University of Murcia, Murcia, Spain;Institute for Biomedical Research of Murcia (IMIB-Arrixaca), Campus de Ciencias de la Salud, Murcia, Spain;Wallenberg Centre for Molecular Medicine, Linköping University, Linköping, Sweden;

关键词: LIF;    LIF receptor;    embryo transfer;    endometrium;    immunotolerance;    allogeneic pregnancy;   

DOI  :  10.3389/fvets.2020.611598

来源: DOAJ

【 摘 要 】

Despite its advantages for pig breeding, embryo transfer (ET) has a major handicap: high embryo mortality during the pre- and implantation period, probably caused by divergent phenomena of tolerance between the immunologically unrelated (i.e., allogeneic) embryos and the recipient sow. Thus, to reach a similar maternal tolerance as in conventional breeding by artificial insemination (AI) would be the key to ET-success. For this reason, we studied the expression of the leukemia inhibitory factor (LIF) cytokine and its receptor in the pig endometrium during the implantation period (days 18 and 24) in sows subjected to ET (AL group) vs. post-cervical-AI controls (Hemi-AL group). Quantification of expression was performed at both mRNA (rt-qPCR) and protein (WB) levels. The expression of endometrial LIF on day 24 was considerably lower in ET than in AI pregnancies. Correlations between endometrial mRNA levels of LIF and LIF-R showed that, contrary to early AI-pregnancies, ET-pregnancies lack an inverse relation between cytokine and receptor levels. In conclusion, ET-pregnancies lack sufficient endometrial levels of LIF to develop adequate immunotolerance mechanisms to prevent the rejection of allogeneic ET-embryos.

【 授权许可】

Unknown