Leukemia inhibitory factor enhances mast cell growth in a mast cell/fibroblast co‐culture system through Stat3 signaling pathway of fibroblasts

FEBS Letters

Morita, Eishin¹  Tanaka, Toshihiko¹  Gyotoku, Eiichi¹  Kameyoshi, Yoshikazu¹  Mihara, Shoji¹  Yamamoto, Shoso¹  Hiragun, Takaaki¹ 

[1] Department of Dermatology, Hiroshima University School of Medicine, Hiroshima, Japan

关键词: Bone marrow-derived mast cell;    Co-culture;    Leukemia inhibitory factor;    Signal transducer and activator of transcription 3;    3T3 fibroblast;    BMMC;    bone marrow-derived mast cells;    ERK;    extracellular signal-regulated kinase;    HA;    hemagglutinin;    IL-6;    interleukin-6;    JAK;    Janus kinase;    LIF;    leukemia inhibitory factor;    LIFR;    LIF receptor;    PBS;    phosphate-buffered saline;    SCF;    stem cell factor;    Stat3;    signal transducer and activator of transcription 3;   

DOI  :  10.1016/S0014-5793(00)02355-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Leukemia inhibitory factor (LIF) enhanced mast cell growth in a mast cell/3T3 fibroblast co-culture system, however the precise mechanisms have not been defined. Western blot analysis showed that bone marrow-derived mast cells failed to express both LIF receptor (LIFR) and gp130, whereas 3T3 fibroblasts expressed both LIFR and gp130. This result indicates that the activity of LIF for mast cell growth is mediated by 3T3 fibroblasts. Signal transducer and activator of transcription (Stat) 3-transfected 3T3 fibroblasts enhanced mast cell growth. In addition, dominant-negative Stat3-transfected fibroblasts blocked LIF-mediated mast cell growth in the co-culture system. In conclusion, LIF-induced mast cell growth in the co-culture system is mediated by an indirect pathway via 3T3 fibroblasts through activating Stat3 signaling pathway in 3T3 fibroblasts.

【 授权许可】

Unknown