Signaling crosstalk underlying synergistic induction of astrocyte differentiation by BMPs and IL‐6 family of cytokines

FEBS Letters

Ochiai, Wataru¹  Yanagisawa, Makoto¹  Arakawa, Hirokazu²  Nakashima, Kinichi¹  Taga, Tetsuya¹  Takizawa, Takumi¹ 

[1] Department of Cell Fate Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1, Honjo, Kumamoto 860-0811, Japan;Department of Pediatrics, School of Medicine, Gunma University, Gunma, Japan

关键词: Astrocyte differentiation;    Bone morphogenetic protein;    Crosstalk;    Interleukin-6 family of cytokine;    Smad;    STAT3;    BMP;    bone morphogenetic protein;    LIF;    leukemia inhibitory factor;    IL;    interleukin;    GFAP;    glial fibrillary acidic protein;    STAT3;    signal transducer and activator of transcription 3;    TGF-β;    transforming growth factor-β;    LIFR;    LIF receptor;    E;    embryonic day;    sIL-6R;    soluble IL-6 receptor;    RT-PCR;    reverse transcription-polymerase chain reaction;    DN-STAT3;    a dominant negative form of STAT3;   

DOI  :  10.1016/S0014-5793(01)02095-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We here show that bone morphogenetic protein (BMP) 7 acted in synergy with the distinct type of cytokines, leukemia inhibitory factor (LIF) and interleukin (IL) 6 that are in the IL-6 family, to induce astrocyte differentiation from neuroepithelial cells as assessed by expression of glial fibrillary acidic protein (GFAP). In this synergistic action, transcription factors, Smads and STAT3 (for signal transducer and activator of transcription 3) activated by respective group of cytokines, as well as a transcriptional coactivator p300 were essential. Taken together with our previous finding that the synergistic astrocyte induction by BMP2 and LIF is attributed to the complex formation of Smads and STAT3 bridged by p300, it is conceivable that this complex formation is a mechanism utilized in common by two different types of cytokines belonging to the BMP and IL-6 families in order to synergistically induce astrocyte differentiation.

【 授权许可】

Unknown