The Effect of Erythropoietin on Neuronal Apoptosis in Hippocampal Dentate Gyrus in Streptozotocin-Induced Alzheimer's Disease in Rats

مجله دانشکده پزشکی اصفهان

卷:35

Bahman Rashidi¹  Parham Reisi²  Moloud Shabrang³  Hojjatallah Alaei⁴  Mohammad Reza Sharifi⁴ 

[1] Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;

[2] Associate Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;

[3] Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;

[4] Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran;

关键词: Alzheimer's disease;    Erythropoietin;    Streptozotocin;    Apoptosis;    Hippocampus;   

DOI  :  10.22122/jims.v35i454.8745

来源: DOAJ

【 摘 要 】

Background: Alzheimer's disease is a disorder associated with neuronal degeneration in the areas involve in learning and memory. It has been proposed that erythropoietin probably has neuroprotective effects. Therefore, the aim of the present study was to evaluate the effect of erythropoietin on neuronal apoptosis in the granular layer of dentate gyrus of the hippocampus in an animal model of Alzheimer's disease.Methods: Animal model of Alzheimer's was established via bilateral intracerebroventricular (ICV) injection of streptozotocin (STZ). After 2 weeks and observation of cognitive disorder, rats received intraperitoneal (IP) injection of erythropoietin every other day with a dose of 5000 IU/kg for 2 weeks. Finally, the rats were anesthetized and their brains were dissected for immunohistochemical study. Terminal deoxynucleotidyl transferase biotin-dUTP Nick-End Labeling (TUNEL) method was used to study neuronal apoptosis.Findings: Intracerebroventricular injection of streptozotocin increased the number of apoptotic cells in the granular layer of dentate gyrus significantly (P < 0.05). Although erythropoietin had no effect on the control rats, it significantly reduced the number of apoptotic cells in the lesion group (P < 0.01)Conclusion: The results of this study indicate that erythropoietin may be effective in reducing the complications of neurodegenerative diseases by reducing neuronal apoptosis.

【 授权许可】

Unknown