Enhancement of Laccase Production from Pleurotus ostreatus PVCRSP-7 by altering the Nutritional Conditions using Response Surface Methodology

BioResources

卷:9

Moses Rajasekara Pandian¹  Potu Venkata Chiranjeevi¹  Sathish Thadikamala² 

[1] Arignar Anna Government Arts College, Namakkal- 637 001. Tamil Nadu, India.;

[2] National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (A Govt of India);

关键词: Lignolytic enzymes;    Response surface methodology;    Laccase;    Optimization;    Submerged fermentation;    Biopress;    Pleurotus ostreatus;   

DOI  :  10.15376/biores.9.3.4212-4225

来源: DOAJ

【 摘 要 】

Submerged culture conditions for laccase production by Pleurotus ostreatus were optimized by response surface methodology (RSM). A total of six factors, carbon (glucose), nitrogen sources (urea and peptone), 2,5-xylidine (inducer), wheat bran (lignocellulosic material), and medium pH, were optimized. A total of 50 experiments were conducted, and the obtained data were modeled using a second-order polynomial. The optimized conditions show significant improvement in laccase expression, by approximately 3.5-fold (12,124 U/L).

【 授权许可】

Unknown