Optimization of laccase production from Marasmiellus palmivorus LA1 by Taguchi method of Design of experiments

BMC Biotechnology

Research Article

Padma Nambisan¹  Nayana Parambayil¹  Anuja Dileep¹  Anala Ramgopal¹  P. S. Soumya¹  Nafeesathul Miziriya¹  M. S. Kiran Lakshmi¹  Aiswarya Chenthamarakshan¹ 

[1] Department of Biotechnology, Cochin University of Science and Technology, Cochin-22, Kerala, India;

关键词: Laccase;    Taguchi DOE;    Solid state fermentation;    Marasmiellus palmivorus;    Optimization;   

DOI  :  10.1186/s12896-017-0333-x

 received in 2016-08-10, accepted in 2017-02-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFungal laccase has profound applications in different fields of biotechnology due to its broad specificity and high redox potential. Any successful application of the enzyme requires large scale production. As laccase production is highly dependent on medium components and cultural conditions, optimization of the same is essential for efficient product production.ResultsProduction of laccase by fungal strain Marasmiellus palmivorus LA1 under solid state fermentation was optimized by the Taguchi design of experiments (DOE) methodology. An orthogonal array (L8) was designed using Qualitek-4 software to study the interactions and relative influence of the seven selected factors by one factor at a time approach. The optimum condition formulated was temperature (28 °C), pH (5), galactose (0.8%w/v), cupric sulphate (3 mM), inoculum concentration (number of mycelial agar pieces) (6Nos.) and substrate length (0.05 m). Overall yield increase of 17.6 fold was obtained after optimization. Statistical optimization leads to the elimination of an insignificant medium component ammonium dihydrogen phosphate from the process and contributes to a 1.06 fold increase in enzyme production. A final production of 667.4 ± 13 IU/mL laccase activity paves way for the application of this strain for industrial applications.ConclusionStudy optimized lignin degrading laccases from Marasmiellus palmivorus LA1. This laccases can thus be used for further applications in different scales of production after analyzing the properties of the enzyme. Study also confirmed the use of taguchi method for optimizations of product production.

【 授权许可】

CC BY   
© The Author(s). 2017