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BioResources
Integration of Artificial Neural Network Modeling and Genetic Algorithm Approach for Enrichment of Laccase Production in Solid State Fermentation by Pleurotus ostreatus
Moses Rajasekara Pandian1  Potu Venkata Chiranjeevi2  Sathish Thadikamala3 
[1] A.A.Government Arts College Namakkal – 637 001.Tamilanadu;Department of Zoology, Arignar Anna Government Arts College, Namakkal- 637 001. Tamil Nadu, India. Present address: National Institute of Nutrition, Tarnaka, Hyderabad, A.P, India;National Institute of Ocean Technology (NIOT), Ministry of Earth Sciences (A Govt of India);
关键词: Laccase;    Artificial intelligence;    Neural networks;    Lignocellulolytic enzyme;    Genetic algorithm;    Optimization;   
DOI  :  10.15376/biores.9.2.2459-2470
来源: DOAJ
【 摘 要 】

Black gram husk was used as a solid substrate for laccase production by Pleurotus ostreatus, and various fermentation conditions were optimized based on an artificial intelligence method. A total of six parameters, i.e., temperature, inoculum concentration, moisture content, CuSO4, glucose, and peptone concentrations, were optimized. A total of 50 experiments were conducted, and the obtained data were modeled by a hybrid of artificial neural network (ANN) and genetic algorithm (GA) approaches. ANN was employed to model the experimental data, and the predicted values were further optimized by GA. Employment of ANN–GA hybrid methodology resulted in a significant improvement, as approximately two-fold laccase production (4244 U/gds) was achieved.

【 授权许可】

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