Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

AMB Express

卷:11

Martin Paul Banda-Magaña¹  Mónica Rebeca Gil-Garzon¹  Jesús Antonio Jáuregui-Jáuregui²  Miguel Flores-Gatica³  Liliana Monserrath Mora-Galvez³  Karla Mayolo-Deloisa³  Cuauhtemoc Licona-Cassani³  Héctor Castañeda-Aponte³  Mario A. Torres-Acosta³ 

[1]BIOMENTUM SAPI de CV

[2]|Tecnologico de Monterrey, Departamento de Bioingeniería

[3]|Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA

关键词: Hyaluronic acid;    Primary recovery;    Aqueous two-phase systems;    Downstream process;    Streptococcus equi subsp. zooepidemicus;   

DOI  :  10.1186/s13568-021-01287-5

来源: DOAJ

【 摘 要 】

Abstract Given its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decades, microbial fermentations have become an important source for the industrial production of HA. However, due to its final applications, microbial HA must undergo critical and long purification processes to ensure clinical and cosmetic grade purity. Aqueous two-phase systems (ATPS) have proven to be an efficient technique for the primary recovery of high-value biomolecules. Nevertheless, their implementation in HA downstream processing has been practically unexplored. In this work, polyethylene glycol (PEG)–citrate ATPS were used for the first time for the primary recovery of HA produced with an engineered strain of Streptococcus equi subsp. zooepidemicus. The effects of PEG molecular weight (MW), tie-line length (TLL), volume ratio (VR), and sample load on HA recovery and purity were studied with a clarified fermentation broth as feed material. HA was recovered in the salt-rich bottom phase, and its recovery increased when a PEG MW of 8000 g mol−1 was used. Lower VR values (0.38) favoured HA recovery, whereas purity was enhanced by a high VR (3.50). Meanwhile, sample load had a negative impact on both recovery and purity. The ATPS with the best performance was PEG 8000 g mol−1, TLL 43% (w/w), and VR 3.50, showing 79.4% HA recovery and 74.5% purity. This study demonstrated for the first time the potential of PEG–citrate ATPS as an effective primary recovery strategy for the downstream process of microbial HA.

【 授权许可】

Unknown