Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol)

Brazilian Archives of Biology and Technology

Leonie Asfora Sarubbo²  Luciana Alves De Oliveira¹  Ana Lúcia Figueiredo Porto¹  Galba Maria De Campos-takaki²  Elias Basile Tambourgi¹ 

[1] ,Universidade Católica de Pernambuco Departamento de Química Recife PE ,Brazil

关键词: Aqueous two-phase systems;    ine serum albumin;    Cashew-nut tree gum;    protein partition;   

DOI  :  10.1590/S1516-89132004000500003

来源: SciELO

【 摘 要 】

The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.

【 授权许可】

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