Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Brazilian Archives of Biology and Technology

Xu Yan²  Maria Aparecida Souza¹  Marcela Z. R. Pontes¹  Michele Vitolo¹  Adalberto Pessoa Júnior¹ 

[1] ,Hebei Institute of Technology Department of Chemical Engineering Tangshan Hebei ,P. R. China

关键词: Affinity partitioning;    Aqueous two-phase systems;    Reactive dyes;    Ligands;    Enzymes;   

DOI  :  10.1590/S1516-89132003000400030

来源: SciELO

【 摘 要 】

From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.

【 授权许可】

CC BY-NC   
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