Brazilian Archives of Biology and Technology | |
Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems | |
Xu Yan2  Maria Aparecida Souza1  Marcela Z. R. Pontes1  Michele Vitolo1  Adalberto Pessoa Júnior1  | |
[1] ,Hebei Institute of Technology Department of Chemical Engineering Tangshan Hebei ,P. R. China | |
关键词: Affinity partitioning; Aqueous two-phase systems; Reactive dyes; Ligands; Enzymes; | |
DOI : 10.1590/S1516-89132003000400030 | |
来源: SciELO | |
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【 摘 要 】
From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phase system with other techniques for more effective purification of enzymes are also presented.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
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RO202005130165130ZK.pdf | 52KB | ![]() |