Affinity chromatography and affinity partition of human serum pre‐albumin using immobilized remazol yellow GGL

FEBS Letters

Birkenmeier, G.¹  Kopperschläger, G.¹  Tschechonien, B.² 

[1] Institute of Physiological Chemistry, Karl-Marx-University, DDR-7010 Leipzig, Liebigstrasse 16, GDR;Department of Biochemistry and Immunochemical Laboratory for Research on Malignant and Embryonal Tissue, 2nd Moscow Medical Institute, Moscow G-435, USSR

关键词: Affinity chromatography;    Affinity partitioning;    Albumin;    Pre-albumin;    Reactive dye;   

DOI  :  10.1016/0014-5793(84)81097-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pre-albumin was isolated from human serum or plasma by ammonium sulphate fractionation and by chromatography on dye-substituted Sephadex G-100 to a high degree of purity. The interaction of the pre-albumin with the dye Remazol Yellow GGL was studied in more detail by means of affinity partitioning in an aqueous two-phase system composed of dextran and poly(ethylene glycol). In the presence of dye-substituted poly(ethylene glycol) the partition coefficient of pre-albumin increases, indicating an interaction with the dye. Human serum albumin was found to enhance this interaction considerably. A similar effect was also shown by bovine albumin but not by egg albumin

【 授权许可】

Unknown