Antibody-Conjugated Magnetic Beads for Sperm Sexing Using a Multi-Wall Carbon Nanotube Microfluidic Device

Micromachines

卷:13

Trisadee Khamlor¹  Chalinee Phiphattanaphiphop²  Komgrit Leksakul²  Thananut Wanta²  Rungrueang Phattanakun³ 

[1] Animal and Aquatic Science Department, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand;

[2] Industrial Engineering Department, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand;

[3] Synchrotron Light Research Institute, Nakhon Ratchasima 30000, Thailand;

关键词: monoclonal antibody conjugated;    sperm sexing;    multi-wall carbon nanotubes;    magnetic beads;    microfluidic;   

DOI  :  10.3390/mi13030426

来源: DOAJ

【 摘 要 】

This study proposes a microfluidic device used for X-/Y-sperm separation based on monoclonal antibody-conjugated magnetic beads, which become positively charged in the flow system. Y-sperms were selectively captured via a monoclonal antibody and transferred onto the microfluidic device and were discarded, so that X-sperms can be isolated and commercially exploited for fertilization demands of female cattle in dairy industry. Therefore, the research team used monoclonal antibody-conjugated magnetic beads to increase the force that causes the Y-sperm to be pulled out of the system, leaving only the X-sperm for further use. The experimental design was divided into the following: Model 1, the microfluid system for sorting positive magnetic beads, which yielded 100% separation; Model 2, the sorting of monoclonal antibody-conjugated magnetic beads in the fluid system, yielding 98.84% microcirculation; Model 3, the sorting of monoclonal antibody-conjugated magnetic beads with sperm in the microfluid system, yielding 80.12% microcirculation. Moreover, the fabrication microfluidic system had thin film electrodes created via UV lithography and MWCNTs electrode structure capable of erecting an electrode wall 1500 µm above the floor with a flow channel width of only 100 µm. The system was tested using a constant flow rate of 2 µL/min and X-/Y-sperm were separated using carbon nanotube electrodes at 2.5 V. The structure created with the use of vertical electrodes and monoclonal antibody-conjugated magnetic beads technique produced a higher effective rejection effect and was able to remove a large number of unwanted sperm from the system with 80.12% efficiency.

【 授权许可】

Unknown