Influence of Magnetic Microparticles Isolation on Adenine Homonucleotides Structure

Materials

Monika Kremplova¹  Dana Fialova¹  Lukas Nejdl¹  David Hynek¹  Libuse Trnkova¹  Jaromir Hubalek¹  Rene Kizek¹ 

[1] Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic; E-Mails:

关键词: adenine;    adenine interaction;    magnetic beads;    square wave voltammetry;    aptamer;    biosensor;    nanobiotechnology;   

DOI  :  10.3390/ma7031455

来源: mdpi

【 摘 要 】

The electroactivity of purine and pyrimidine bases is the most important property of nucleic acids that is very useful for determining oligonucleotides using square wave voltammetry. This study was focused on the electrochemical behavior of adenine-containing oligonucleotides before and after their isolation using paramagnetic particles. Two peaks were detected—peak A related to the reduction of adenine base and another peak B involved in the interactions between individual adenine strands and contributes to the formation of various spatial structures. The influence of the number of adenine bases in the strand in the isolation process using paramagnetic particles was investigated too.

【 授权许可】

CC BY   
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