Aptamer-Modified Magnetic Beads in Biosensing

Sensors

卷:18

Harshvardhan Modh¹  Johanna-Gabriela Walter¹  Thomas Scheper¹ 

[1] Institute of Technical Chemistry, Leibniz University of Hannover, Hannover 30167, Germany;

关键词: aptamer;    magnetic beads;    analytical applications;    electrochemical assays;    optical assays;    point-of-care-testing;   

DOI  :  10.3390/s18041041

来源: DOAJ

【 摘 要 】

Magnetic beads (MBs) are versatile tools for the purification, detection, and quantitative analysis of analytes from complex matrices. The superparamagnetic property of magnetic beads qualifies them for various analytical applications. To provide specificity, MBs can be decorated with ligands like aptamers, antibodies and peptides. In this context, aptamers are emerging as particular promising ligands due to a number of advantages. Most importantly, the chemical synthesis of aptamers enables straightforward and controlled chemical modification with linker molecules and dyes. Moreover, aptamers facilitate novel sensing strategies based on their oligonucleotide nature that cannot be realized with conventional peptide-based ligands. Due to these benefits, the combination of aptamers and MBs was already used in various analytical applications which are summarized in this article.

【 授权许可】

Unknown