Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG

Asian Pacific Journal of Tropical Biomedicine

卷:7

Daymí Serpa¹  Yanely Tirado¹  Yolanda Valdes¹  José Luis Pérez¹  Reinaldo Acevedo¹  Luis Izquierdo¹  Reinier Borrero¹  Nadine Alvarez¹  Rubén Cabrera¹  Sonsire Fernández¹  Caridad Zayas¹  Armando Acosta²  Mohd-Nor Norazmi³  María Elena Sarmiento³  Ramlah Kadir³ 

[1] Finlay Institute, Ave. 27, No. 19805, P.O. Box 16017, La Lisa, Havana CP11600, Cuba;

[2] Institute for Research in Molecular Medicine, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;

[3] School of Health Sciences, University Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia;

关键词: BCG;    Immunogenicity;    Mycobacterium tuberculosis;    Proteoliposomes;   

DOI  :  10.1016/j.apjtb.2016.12.013

来源: DOAJ

【 摘 要 】

Objective: To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis (M. tuberculosis) of a proteoliposome (PL) from Mycobacterium bovis Bacillus Calmette–Guérin (BCG) with and without alum hydroxide (AL) as adjuvant (PLBCG-AL and PLBCG, respectively) in BALB/c mice.Methods: BALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immunoglobulin G (IgG), IgG1 and IgG2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western blot.Results: Sera from animals immunized with PLBCG-AL and PLBCG showed significant increase in specific total IgG and IgG1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in vivo.Conclusions: The cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the protective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages.

【 授权许可】

Unknown