Altered gut microbiota composition with antibiotic treatment impairs functional recovery after traumatic peripheral nerve crush injury in mice: effects of probiotics with butyrate producing bacteria

BMC Research Notes

卷:15

Andrew Rodenhouse¹  Grant D. Wandling¹  John P. Hegarty¹  M. A. Hassan Talukder¹  Kelsey Lloyd¹  Kristen M. Manto¹  Prem Kumar Govindappa¹  Mary O’Brien¹  John C. Elfar¹  Jung Il Lee²  Justin R. Wright³  Jeremy R. Chen See³  Regina Lamendella³  Samantha L. Anderson³ 

[1] Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science, The Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center;

[2] Department of Orthopedic Surgery, Korea University Guro Hospital;

[3] Wright Labs LLC;

关键词: Antibiotics;    Probiotics;    16S rRNA analysis;    Gut microbiota;    Peripheral nerve injury;    Functional recovery;   

DOI  :  10.1186/s13104-022-05967-8

来源: DOAJ

【 摘 要 】

Abstract Objective Antibiotics (ABX) are widely used for life-threatening infections and also for routine surgical operations. Compelling evidence suggests that ABX-induced alterations of gut microbiota composition, termed dysbiosis, are linked with diverse disease states including neurological and neurodegenerative conditions. To combat the consequences of dysbiosis, probiotics (PBX) are widely used. ABX-induced dysbiosis is reported to impair neurological function after spinal cord injury. Traumatic peripheral nerve injury (TPNI) results in profound neurologic impairment and permanent disability. It is unknown whether ABX treatment-induced dysbiosis has any impact on TPNI-induced functional recovery, and if so, what role medical-grade PBX could have on TPNI recovery. Results In this study, ABX-induced dysbiosis and PBX-induced microbiota enrichment models were used to explore the potential role of gut microbiome in TPNI. Stool analysis with 16S ribosomal RNA (rRNA) gene sequencing confirmed ABX-induced dysbiosis and revealed that ABX-induced changes could be partially restored by PBX administration with an abundance of butyrate producing bacteria. Pre-injury ABX significantly impaired, but pre-injury PBX significantly improved post-TPNI functional recovery. Importantly, post-injury PBX protected against pre-injury ABX-induced functional impairment. These findings demonstrate that reestablishment of gut microbiota composition with butyrate producing PBX during ABX-induced dysbiosis could be a useful adjuvant therapy for TPNI.

【 授权许可】

Unknown