Phylogeny of Vibrio vulnificus from the Analysis of the Core-Genome: Implications for Intra-Species Taxonomy

Frontiers in Microbiology

卷:8

Yael Danin-Poleg¹  Yechezkel Kashi¹  Cynthia J. Gibas²  Shatavia S. Morrison²  Paul A. Gulig³  Carlos Llorens⁴  Francisco J. Roig⁴  Fernando González-Candelas⁵  Craig Baker-Austin⁶  Edward J. Feil⁸  Belén Fouz⁹  Carmen Amaro⁹  Eva Sanjuán⁹  James D. Oliver¹⁰ 

[1]0Faculty of Biotechnology and Food Engineering, Technion–Israel Institute of Technology, Haifa, Israel

[2]|1Department of Bioinformatics and Genomics, the University of North Carolina at Charlotte, Charlotte, NC, United States

[3]|2Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL, United States

[4]|Biotechvana, Parc Cientific, Universitat de Valencia, Valencia, Spain

[5]|CIBEResp, National Network Center for Research on Epidemiology and Public Health, Instituto de Salud Carlos III, Valencia, Spain

[6]|Centre for Environment, Fisheries and Aquaculture Science, Weymouth, United Kingdom

[7]|Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States

[8]|Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom

[9]|Departmento de Microbiología y Ecología, Universidad de Valencia, Valencia, Spain

[10]|Duke University Marine Lab, Beaufort, NC, United States

[11]|Estructura de Investigación Interdisciplinar en Biotecnología y Biomedicina BIOTECMED, University of Valencia, Valencia, Spain

[12]|Joint Research Unit on Infection and Public Health FISABIO-Salud Pública and Universitat de Valencia-I2SysBio, Valencia, Spain

关键词: microbial evolution;    pathogens;    SNP;    Vibrio vulnificus;    core genome;    virulence plasmid;   

DOI  :  10.3389/fmicb.2017.02613

来源: DOAJ

【 摘 要 】

Vibrio vulnificus (Vv) is a multi-host pathogenic species currently subdivided into three biotypes (Bts). The three Bts are human-pathogens, but only Bt2 is also a fish-pathogen, an ability that is conferred by a transferable virulence-plasmid (pVvbt2). Here we present a phylogenomic analysis from the core genome of 80 Vv strains belonging to the three Bts recovered from a wide range of geographical and ecological sources. We have identified five well-supported phylogenetic groups or lineages (L). L1 comprises a mixture of clinical and environmental Bt1 strains, most of them involved in human clinical cases related to raw seafood ingestion. L2 is formed by a mixture of Bt1 and Bt2 strains from various sources, including diseased fish, and is related to the aquaculture industry. L3 is also linked to the aquaculture industry and includes Bt3 strains exclusively, mostly related to wound infections or secondary septicemia after farmed-fish handling. Lastly, L4 and L5 include a few strains of Bt1 associated with specific geographical areas. The phylogenetic trees for ChrI and II are not congruent to one another, which suggests that inter- and/or intra-chromosomal rearrangements have been produced along Vv evolution. Further, the phylogenetic trees for each chromosome and the virulence plasmid were also not congruent, which also suggests that pVvbt2 has been acquired independently by different clones, probably in fish farms. From all these clones, the one with zoonotic capabilities (Bt2-Serovar E) has successfully spread worldwide. Based on these results, we propose a new updated classification of the species based on phylogenetic lineages rather than on Bts, as well as the inclusion of all Bt2 strains in a pathovar with the particular ability to cause fish vibriosis, for which we suggest the name “piscis.”

【 授权许可】

Unknown