Experimental Evidence of the Long-Term Survival of Infective African Swine Fever Virus Strain Ba71V in Soil under Different Conditions

Pathogens

卷:11

Bronislav Simek¹  Petr Vaclavek¹  Lenka Kavanova²  Romana Moutelikova²  Sarka Kobzova²  Jana Prodelalova²  Jiri Salat²  Magdalena Krasna²  Petra Vasickova² 

[1] State Veterinary Institute Jihlava, Rantirovska 20, 586 01 Jihlava, Czech Republic;

[2] Veterinary Research Institute, Hudcova 70, 602 00 Brno, Czech Republic;

关键词: ASFV;    field isolates;    stability;    hemadsorption;    infectivity testing;   

DOI  :  10.3390/pathogens11060648

来源: DOAJ

【 摘 要 】

The survival of African swine fever virus (ASFV) on different matrices and its infectivity in wild as well as domestic swine is still a matter of interest. ASFV is resistant to environmental effects; this fact is enhanced by the presence of organic material. Therefore, the aim of this work was to determine the ability of laboratory ASFV to survive in soil at different temperatures (4 and 22 °C) and with and without the presence of blood using culture procedures. The suitability of the procedure for determining the viability and titre of the ASFV field strain by the hemadsorption method was also verified, when a higher decrease in virus infectivity in the case of clay compared with peat was demonstrated. The stability of the virus was clearly temperature-dependent, the infectious virus was detected after 112 days, and the viral DNA was still detected in the matrix 210 days after inoculation in a relatively high and stable concentration (between 10⁶ and 10⁷ genome equivalents/mL). Based on this knowledge, soil and other environmental samples could provide rapid and reliable information on the disease outbreak and serve as indicators of the risk posed by the affected locality.

【 授权许可】

Unknown