Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro

Veterinarski Glasnik

Nišavić Jakov¹  Milić Nenad¹ 

[1] Katedra za mikrobiologiju, Fakultet veterinarske medicine, Beograd;

关键词: Newcastle Disease virus;    Vero cells;    hemadsorption;    hemadsorption inhibition;   

DOI  :  10.2298/VETGL0606299N

来源: DOAJ

【 摘 要 】

The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum.

【 授权许可】

Unknown