Genomic Diversity among Equine Herpesvirus-4 Field Isolates

Journal of Veterinary Medical Science

Ken MAEDA¹  Tomio MATSUMURA²  Kazushige KAI¹ 

[1] Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University;Epizootic Research Station, Equine Research Institute, Japan Racing Association

关键词: epizootiology;    equine herpesvirus-4;    field isolates;    genomic diversity;   

DOI  :  10.1292/jvms.67.555

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Cited-By(3)Infection with equine herpesvirus-4 (EHV-4) is a major cause of respiratory tract disease, equine rhinopneumonitis, in horses. Although the full sequence of EHV-4 has been reported, genomic differences among EHV-4 field isolates have not yet been characterized. In this study, the genomic diversity between 23 Japanese EHV-4 isolates was analyzed by digestion with restriction endonucleases (BamHI, BgIII, EcoRI, SacI, and SalI) and polymerase chain reaction (PCR). The restriction endonuclease digestion patterns of the EHV-4 field isolates showed distinct differences which included mobility shifts of some fragments as well as loss and/or gain of fragments. Two EHV-4 genes containing repeat sequences, ORFs 24 and 71, were amplified by PCR and the amplified fragments were compared among the field isolates. The sizes of the amplified fragments varied among epizootiologically unrelated isolates, while the fragments of related isolates had the same size. The observed genomic diversity among EHV-4 field isolates may be a useful tool for epidemiological study of equine rhinopneumonitis by EHV-4 infection.

【 授权许可】

Unknown