Dosage‐dependent effects of monensin on the rumen microbiota of lactating dairy cattle

MicrobiologyOpen

卷:8

Frank Mitloehner¹  Sara Place²  Jeffrey Palumbo³  Jeffery A. McGarvey³  Robert Hnasko³ 

[1]Department of Animal Science University of California Davis California

[2]|National Cattlemen’s Association Sustainable Beef Production Research Centennial Colorado

[3]|United States Department of Agriculture Agricultural Research Service Albany California

关键词: 16S rRNA;    dairy cattle;    microbiota;    monensin;    Rumen;   

DOI  :  10.1002/mbo3.783

来源: DOAJ

【 摘 要 】

Abstract We examined the dose‐dependent effects of feeding lactating dairy cows a standard diet supplemented with monensin at 175, 368, or 518 mg cow‐1 day‐1on the rumen microbiota. For each dosage, 3 animals were randomly assigned into groups and fed the same basal total mixed ration diet supplemented with monensin, at the respective dose. After 20 days, rumen samples were taken and the effect on the microbiota was examined by 16S rRNA gene sequence analysis and qPCR. At the lowest dose no significant change in 16S rRNA gene sequences associated with any bacterial phyla was observed; however, at the medium and high dosages, we observed significant reductions in sequences associated with gram‐positive bacteria and significant increases in those associated with gram‐negative bacteria that were dosage dependent. All dosages reduced the levels of sequences associated with methanogenic archaea in the rumen, with the medium dosage showing the largest decline. No significant difference was observed for the 18S rRNA gene sequences associated with protozoa in any of the libraries. Our results indicate that with this diet the medium dosage of monensin was most efficacious for the reduction in methanogenic archaea in the rumen of lactating dairy.

【 授权许可】

Unknown