Detection of Campylobacter jejuni in rectal swab samplesfrom Rousettus amplexicaudatus in the Philippines

Journal of Veterinary Medical Science

Satoshi TANIGUCHI⁴  Shigeru KYUWA⁸  Ken MAEDA⁷  Yukie KATAYAMA²  Yuki HATTA²  Shinobu TSUCHIAKA²  Edison COSICO³  Tetsuya MIZUTANI²  Tsutomu OMATSU²  Eduardo ERES³  Roberto PUENTESPINA Jr⁹  Yasuhiro YOSHIKAWA⁵  Yumi UNE⁶  Joseph S MASANGKAY¹ 

[1] College of Veterinary Medicine, University of the Philippine Los Baños, 4031 Los Baños, Laguna, Philippines;Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3�?5�?8 Saiwai-cho, Fuchu-shi, Tokyo 183�?8509, Japan;Museum of Natural History, University of the Philippines Los Baños, 4031 Los Baños, Laguna, Philippines;Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, 4�?7�?1 Gakuen, Musashimurayama, Tokyo 208�?0011, Japan;Department of Animal Risk Management, Chiba Institute of Science, Choshi, Chiba 288�?0025, Japan;Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1�?17�?1 Fuchinobe, Sagamihara, Kanagawa 252�?5201, Japan;Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677�?1 Yoshida, Yamaguchi 753�?8515, Japan;Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1�?1�?1 Yayoi, Bunkyo-ku, Tokyo 113�?8657, Japan;University of the Philippines Mindanao, Bago-Oshiro, Davao, Philippines

关键词: 16S rRNA;    bat;    Camplyobacter;    metagenome;    microbiota;   

DOI  :  10.1292/jvms.15-0621

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Bats are the second diversity species of mammals and widely distributed in the world.They are thought to be reservoir and vectors of zoonotic pathogens. However, there isscarce report of the evidence of pathogenic bacteria kept in bats. The precise knowledgeof the pathogenic bacteria in bat microbiota is important for zoonosis control. Thus,metagenomic analysis targeting the V3-V4 region of the 16S rRNA of the rectal microbiotain Rousettus amplexicaudatus was performed using high throughputsequencing. The results revealed that 103 genera of bacteria includingCamplyobacter were detected. Campylobacter was secondpredominant genus, and Campylobacter coli and Campylobacterjejuni were identified in microbiome of R. amplexicaudatus.Campylobacteriosis is one of the serious bacterial diarrhea in human, and the most oftenimplicated species as the causative agent of campylobacteriosis is C.jejuni. Therefore, we investigated the prevalence of C. jejuniin 91 wild bats with PCR. As a result of PCR assay targeted on 16S-23S intergenic spacer,partial genome of C. jejuni was detected only in five R.amplexicaudatus. This is the first report that C. jejuni wasdetected in bat rectal swab samples. C. jejuni is the most common causeof campylobacteriosis in humans, transmitted through water and contact with livestockanimals. This result indicated that R. amplexicaudatus may be a carrierof C. jejuni.

【 授权许可】

Unknown