Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani

International Journal of Molecular Sciences

卷:21

Reinhard Wimmer¹  TeisEsben Sondergaard¹  KåreLehmann Nielsen¹  Trine Sørensen¹  Celine Petersen¹  EvaMia Lang Spedtsberg²  SambaEvelyne Kabemba Kaniki²  TobiasBruun Pedersen²  Wafaa Yasmine²  Jens Muff²  Rikke Matthiesen²  JensLaurids Sørensen²  MikkelRank Nielsen²  SebastianBirkedal Kristensen² 

[1] Aalborg University Aalborg, Department of Chemistry and Bioscience, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark;

[2] Aalborg University Esbjerg, Department of Chemistry and Bioscience, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark;

关键词: polyketides;    yeast;    heterologous expression;    Fusarium;    pigments;    fungi;   

DOI  :  10.3390/ijms21207601

来源: DOAJ

【 摘 要 】

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

【 授权许可】

Unknown