Recombinant yeast VDAC2: a comparison of electrophysiological features with the native form

FEBS Open Bio

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[1] Department of Bioenergetics, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan, Poland;Department of Biological, Geological and Environmental Sciences, Section of Molecular Biology, University of Catania, Italy;Department of Biological, Geological and Environmental Sciences, Section of Molecular Biology, University of Catania, Italy;National Institute for Biomembranes and Biosystems, Section of Catania, Italy;Department of Biomedical and Biotechnological Sciences, University of Catania, Italy;Department of Biomedical and Biotechnological Sciences, University of Catania, Italy;Department of Biological, Geological and Environmental Sciences, Section of Molecular Biology, University of Catania, Italy;Department of Biomedical and Biotechnological Sciences, University of Catania, Italy;National Institute for Biomembranes and Biosystems, Section of Catania, Italy;

关键词: electrophysiology;    heterologous expression;    mitochondria;    mitochondrial porins;    planar lipid bilayer;    VDAC;    yeast;    VDAC;    yVDAC;   

DOI  :  10.1002/2211-5463.12574

来源: publisher

【 摘 要 】

Voltage‐dependent anion channel isoform 2 of the yeast Saccharomyces cerevisiae (yVDAC2) was believed for many years to be devoid of channel activity. Recently, we isolated yVDAC2 and showed that it exhibits channel‐forming activity in the planar lipid bilayer system when in its so‐called native form. Here, we describe an alternative strategy for yVDAC2 isolation, through heterologous expression in bacteria and refolding in vitro. Recombinant yVDAC2, like its native form, is able to form voltage‐dependent channels. However, some differences between native and recombinant yVDAC2 emerged in terms of voltage dependence and ion selectivity, suggesting that, in this specific case, the recombinant protein might be depleted of post‐translational modification(s) that occur in eukaryotic cells.

【 授权许可】

Unknown