Toxicity evaluation of water extract of tissue-cultured Taraxacum formosanum by acute, subacute administration, and Ames test

Electronic Journal of Biotechnology

卷:45

Yi-Han Tseng¹  Hsiao-Wei Wen²  Jiunn-Wang Liao³  Hsin-Yi Yin³  Wen-Che Tsai³  Hung-Chi Chang⁴  Dinesh Chandra Agrawal⁵ 

[1] Corresponding authors.;

[2] Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan, ROC;

[3] Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan, ROC;

[4] Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung 41349, Taiwan, ROC;

[5] Graduate Institute of Veterinary Pathology, National Chung Hsing University, Taichung 40227, Taiwan, ROC;

关键词: Acute toxicity;    Ames test;    Antiproliferative;    Dandelion;    Food-safety;    Herbal medicine;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: Taraxacum species (commonly known as dandelion) used as herbal medicine have been reported to exhibit an antiproliferative effect on hepatoma cells and antitumor activity in non-small-cell lung cancer cells. Although several investigations have demonstrated the safety of Taraxacum officinale, the safety of tissue-cultured plants of T. formosanum has not been assessed so far. Therefore, the present study examines the safety of the water extract of the entire plant of tissue cultured T. formosanum based on acute and subacute toxicity tests in rats, as well as the Ames tests. Results: No death or toxicity symptoms were observed in the acute and subacute tests. The results of the acute test revealed that the LD50 (50% of lethal dose) value of the T. formosanum water extract for rats exceeded 5 g/kg bw. No abnormal changes in the body weight, weekly food consumption, organ weight, or hematological, biochemical, and morphological parameters were observed in the subacute toxicity test. Thus, the no observed adverse effect level (NOAEL) of T. formosanum water extract was estimated to be higher than 2.0 g/kg. Finally, the results of the Ames test revealed that T. formosanum water extract was not genotoxic at any tested concentration to any of five Salmonella strains. Conclusions: The water extract of tissue-cultured T. formosanum was non-toxic to rats in acute and subacute tests and exhibited no genotoxicity to five Salmonella strains.How to cite: Tsai WC, Chang HC, Tseng YH, et al. Toxicity evaluation of water extract of tissue-cultured Taraxacum formosanum by acute, subacute administration, and Ames test. Electron J Biotechnol 2020;45. https://doi.org/10.1016/j.ejbt.2020.04.001

【 授权许可】

Unknown