Evaluation of a water extract of So-Cheong-Ryong-Tang for acute toxicity and genotoxicity using in vitro and in vivo tests

BMC Complementary and Alternative Medicine

Hyeun-Kyoo Shin¹  Ji-Young Kim²  Chang-Sebo Seo¹  Mee-Young Lee¹ 

[1] Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon 305-811, South Korea;Division of Nonclinical Studies, Korea Institute of Toxicology, 19 Sinseongro, Yuseong-gu, Daejeon 305-343, South Korea

关键词: Acute toxicity;    Micronucleus assay;    Chromosome aberration assay;    Ames test;    So-Cheong-Ryong-Tang;   

Others  :  1219998
DOI  :  10.1186/s12906-015-0737-x

 received in 2015-02-10, accepted in 2015-06-22,  发布年份 2015

【 摘 要 】

Background

So-Cheong-Ryong-Tang, a traditional Korean medicine, has been used empirically for the treatment of asthma, allergic rhinitis, and colds for hundreds of years. However, its genotoxicity has been rarely examined.

Methods

We therefore investigated the genotoxicity of an aqueous extract of So-Cheong-Ryong-Tang (SCRT) in two in vitro and one in vivo assays: a bacterial reverse mutation assay (Ames test), a chromosomal aberration assay, and a micronucleus assay, respectively.

Results

In the bacterial reverse mutation assay, SCRT did not increase revertant colony numbers in Salmonella. typhimurium strains (TA98, TA100, TA1535, and TA1537) or an Escherichia coli strain (WP2 uvrA) regardless of metabolic activation or the duration of treatment. However, statistically significant differences in the incidence of chromosomal aberrations following SCRT >4000 μg/mL were observed in Chinese hamster lung cells exposed with or without an S9 enzyme and cofactor mixture.

Conclusions

These results suggest further genotoxic testing of SCRT, such as a comet assay, to ascertain its generally recognized safety.

【 授权许可】

   
2015 Lee et al.

【 图 表 】

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