Study on acute toxicity of amoxicillin wastewater to Zebrafish

1st International Global on Renewable Energy and Development

Xie, Weifang^1,2 ; Shen, Hongyan^1,2

College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang

050018, China^1

Pharmaceutical Molecular Chemistry Laboratory of Hebei Province, Hebei University of Science and Technology, Shijiazhuang

050018, China^2

关键词: Acute toxicity;    Concentration ranges;    Experimental methods;    Exposure concentration;    Lethal concentration;    Observation Period;    Pharmaceutical wastewater;    Volume percentage;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/100/1/012162/pdf
DOI  :  10.1088/1755-1315/100/1/012162

来源: IOP

【 摘 要 】

The main research in this paper is to obtain the effect of pharmaceutical wastewater on the acute toxicity of Zebrafish. The experimental method of exposure is used in this research. Experiments were carried out with different groups of pharmaceutical wastewater. Zebrafish was cultivated in a five liter fish tank. In the experiment, according to mortality, initially a 96h preliminary test was carried out at exposure concentrations to determine if the amoxicillin wastewater was toxic and to define the concentration range (24h LC100, 96h LC0) to be employed in the definitive tests. Based on the half lethal concentration of Zebrafish, the acute toxicity of amoxicillin wastewater to Zebrafish was calculated and the toxicity grade of wastewater was determined. In the experiment, the Zebrafish was exposed with amoxicillin wastewater during 96h. The 24h, 48h, 72h and 96h LC50of amoxicillin wastewater on the Zebrafish were 63.10%, 53.70%, 41.69% and 40.74%, respectively. At 96h, the test time is the longest, and the value of LC50is the smallest. In the observation period of 96 hours, the LC50of amoxicillin wastewater were in the range of 40% ∼ 60% and the value of Tua is 1 ∼ 2. It indicates amoxicillin wastewater is low toxic wastewater when the experimental time is shorter than 48h, amoxicillin wastewater is moderate toxicity wastewater when the experimental time is higher than 48h. According to the experimental data, with the exposure time and the volume percentage of amoxicillin wastewater increases, the mortality rate of Zebrafish is gradually increased and the toxicity of amoxicillin wastewater increases. It indicates that the toxicity of amoxicillin wastewater is the biggest and the effect of wastewater on Zebrafish is greatest. In some ways, the toxicity of amoxicillin wastewater can be affected by the test time.