Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions

STAR Protocols

卷:2

Britta Engelhardt¹  Eric V. Shusta²  Benjamin D. Gastfriend²  Sean P. Palecek²  Pelin Kasap³  Hideaki Nishihara³ 

[1] Department of Neurological Surgery, University of Wisconsin–Madison, Madison, WI 53792, USA;

[2] Department of Chemical and Biological Engineering, University of Wisconsin–Madison, Madison, WI 53706, USA;

[3] Theodor Kocher Institute, University of Bern, Freiestrasse 1, 3012 Bern, Switzerland;

关键词: Cell culture;    Immunology;    Neuroscience;    Stem Cells;    Cell Differentiation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion.For complete details on the use and execution of this protocol, please refer to Lian et al. (2014), Nishihara et al. (2020a).

【 授权许可】

Unknown