Design of an Innovative Methodology for Cerebrospinal Fluid Analysis: Preliminary Results

Sensors

卷:21

Andrea Palombi¹  Arnaldo D’Amico¹  Alessandro Zompanti²  Giorgio Pennazza²  Marco Santonico³  Simone Grasso³  NicolaBiagio Mercuri⁴  Tommaso Schirinzi⁴  Alberto Cordella⁵ 

[1] Department of Electronic Engineering, University of Roma Tor Vergata, 00133 Rome, Italy;

[2] Department of Engineering, Campus Bio-Medico, University of Rome Italy, 00128 Rome, Italy;

[3] Department of Science and Technology for Humans and the Environment, Campus Bio-Medico, University of Rome Italy, 00128 Rome, Italy;

[4] Department of Systems Medicine, University of Roma Tor Vergata, 00133 Rome, Italy;

[5] IRCCS Fondazione Santa Lucia, Via Ardeatina 306, 00179 Rome, Italy;

关键词: CSF;    voltammetry;    electrochemical analysis;    biomarkers;    neurodegenerative diseases;   

DOI  :  10.3390/s21113767

来源: DOAJ

【 摘 要 】

Cerebrospinal fluid (CSF) analysis supports diagnosis of neurodegenerative diseases (NDs), however a number of issues limits its potentialities in clinical practice. Here, a newly developed technique for fluid voltammetry, relying on a simple sensor (BIOsensor-based multisensorial system for mimicking Nose, Tongue and Eyes, BIONOTE), was used to test the applicability for CSF analysis. BIONOTE was initially calibrated on an artificial CSF-like solution and then applied on human CSF, either immediately after collection or after refrigerated storage. Following optimization, it was used to evaluate 11 CSF samples correlating the electrochemical dataset with CSF routine parameters and biomarkers of neurodegeneration. Multivariate data analysis was performed for model elaboration and calibration using principal component analysis and partial least squares discriminant analysis. BIONOTE presented a high capacity to predict both physiological and pathological constituents of artificial CSF. It differentiated distinct fresh human CSF samples well but lost accuracy after refrigerated storage. The electrochemical analysis-derived data correlated with either CSF routine cytochemical indexes or a biomarker of neurodegeneration. BIONOTE resulted as being a reliable system for electrochemical analysis of CSF. The CSF fingerprint provided by the sensor has shown itself to be sensitive to CSF modification, thus it is potentially representative of CSF alteration. This result opens the way to its testing in further study addressed at assessing the clinical relevance of the methodology. Because of its advantages due to the ease and rapidity of the methodology, a validation study is now required to translate the technique into clinical practice and improve diagnostic workup of NDs.

【 授权许可】

Unknown