BMC Complementary and Alternative Medicine | 卷:12 |
Synergetic downregulation of 67 kDa laminin receptor by the green tea ( |
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关键词: Cancer; EGCG; IPEC-J2; miRNA; RNA interference; | |
DOI : 10.1186/1472-6882-12-258 | |
来源: DOAJ |
【 摘 要 】
Abstract
Background
In traditional Chinese medicine, green tea is considered to have a life-prolonging effect, possibly as a result of its rich content of antioxidant tea polyphenols, and hence has the potential to prevent cancer. This study investigated the role of the major tea secondary plant compound epigallocatechin gallate (EGCG) for its inhibitory effects on the metastasis-associated 67 kDa laminin receptor (67LR).
Methods
To clarify the impact of EGCG on siRNA-silenced expression of 67LR, we applied an adenoviral-based intestinal
Results
We report co-regulation of EGCG and 67LR, which is known to be an EGCG receptor. siRNA selectively and highly significantly suppressed expression of 67LR under the impact of EGCG in a synergetic manner.
Conclusions
Our findings suggest that 67LR expression is regulated by EGCG via a negative feedback loop. The explicit occurrence of this effect in synergy with a small RNA pathway and a plant-derived drug reveals a new mode of action. Our findings may help to provide insights into the many unsolved health-promoting activities of other natural pharmaceuticals.
【 授权许可】
Unknown