Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS

Molecules

卷:21

Yin Cen¹  Xiai Yang²  Juan Leng²  Liping Liao²  Liangliang Liu²  Aiping Xiao²  Lei Ma³ 

[1]College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

[2]|Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China

[3]|State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China

关键词: bovine serum albumin;    HPLC-MS;    ligands;    magnetic nanoparticles;    Radix astragali;   

DOI  :  10.3390/molecules21111471

来源: DOAJ

【 摘 要 】

Radix astragali is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from the n-butanol part of a Radix astragali extract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from the n-butanol part of a Radix astragali extract and identified as genistin (1), calycosin-7-O-β-d-glucoside (2), ononin (3) and formononetin (4). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation.

【 授权许可】

Unknown