Solid Culturing of Bacillus amyloliquefaciens for α-Amylase Production

Food Technology and Biotechnology

卷:44

Dhanya Gangadharan¹  Kesavan Madhavan Nampoothiri¹  Ashok Pandey¹  Swetha Sivaramakrishnan¹ 

[1] Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695019, India;

关键词: α-amylase;    Bacillus amyloliquefaciens;    solid-state fermentation;    agroresidues;   

DOI  :  

来源: DOAJ

【 摘 要 】

Fourteen different agroresidues were screened for alpha amylase production using Bacillus amyloliquefaciens ATCC 23842. Among them, wheat bran (WB) and groundnut oil cake (GOC) in mass ratio of 1:1 was proved as the best substrate source. Supplementation with 0.01 M KH2PO4 and 1 % soluble starch enhanced the enzyme yield considerably. Maximum enzyme recovery from the solid mass was obtained when extracted with 0.1 M acetate buffer, pH=5.0. Maximum enzyme titer expressed as units per mass of dry substrate obtained was 62 470 U/g after 72 hours of fermentation at 37 °C by using the above solid substrate mixture (5 g) with the initial moisture of 85 % and inoculated with Bacillus amyloliquefaciens of 2·109 CFU/mL.

【 授权许可】

Unknown