Food Technology and Biotechnology | |
α-Amylase Production by Bacillus amyloliquefaciens Using Agro Wastes as Feed Stock | |
Dhanya Gangadharan1  Ashok Pandey1  K. Madhavan Nampoothiri1  | |
[1] Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, 695 019 Trivandrum, India; | |
关键词: α-amylase; Bacillus amyloliquefaciens; fermentor; bioreactor; aeration; submerged fermentation; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
The productivity of enzyme fermentations depends critically on maintaining a high oxygen transfer rate to satisfy the optimal oxygen demand of the microorganism for product formation. Among the several factors that affect oxygen transfer rates in a fermentor are the air flow rate and agitation. The production of α-amylase by Bacillus amyloliquefaciens was performed in 600-mL and 5-litre fermentor with a working volume of 300 mL and 3 L, respectively. The experiments indicated a requirement of high rates of aeration to enhance the enzyme yield. The biomass yield and productivity of the enzyme were found to have a linear relationship with the air flow rate, and the highest productivity was observed at 1.0 vvm. A maximum productivity of 41.4 U/(mL·h) was obtained after 14 h of fermentation in 600-mL fermentor system and a comparable productivity of 40 U/(mL·h) was obtained after 12 h in the 5-litre fermentor.
【 授权许可】
Unknown