Function of phosphoenolpyruvate carboxykinase in mammary gland epithelial cells[S]

Journal of Lipid Research

卷:52

Jianqi Yang¹  Chang-Wen Hsieh²  Maria Hatzoglou²  Michelle Puchowicz²  Michelle Beidelschies²  Charles Huang²  Ilya Bederman³  Colleen M. Croniger⁴ 

[1] Department of Pharmacology, University of Iowa, Carver College of Medicine, Iowa City, IA 52242;

[2] Departments of Nutrition;

[3] Pediatrics, Case Western Reserve University, Cleveland, OH 44106;

[4] To whom correspondence should be addressed [e-mail];

关键词: adipocytes;    gluconeogenesis;    glyceroneogenesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Previously, we have shown that Pck1 expression in mammary gland adipocytes and white adipose tissue maintains triglyceride stores through glyceroneogenesis, and these lipids were used for synthesis of milk triglycerides during lactation. Reduced milk triglycerides during lactation resulted in patterning of the newborn for insulin resistance. In this study, the role of Pck1 in mammary gland epithelial cells was analyzed. The developmental expression of Pck1 decreased in isolated mouse mammary gland epithelial cells through development and during lactation. Using HC11, a clonal mammary epithelial cell line, we found that both Janus kinase 2 signal transducers and activators of transcription 5 and the AKT pathways contributed to the repression of Pck1 mRNA by prolactin. These pathways necessitate three accessory factor regions of the Pck1 promoter for repression by prolactin. Using [U-13C6]glucose, [U-13C3]pyruvate, and [U-13C3]glycerol in HC11 cells, we determined that Pck1 functions in the pathway for the conversion of gluconeogenic precursors to glucose and contributes to glycerol-3-phosphate synthesis through glyceroneogenesis. Therefore, Pck1 plays an important role in both the mammary gland adipocytes and epithelial cells during lactation.

【 授权许可】

Unknown