Non-Classical Gluconeogenesis-Dependent Glucose Metabolism in Rhipicephalus microplus Embryonic Cell Line BME26

International Journal of Molecular Sciences

Renato Martins da Silva²  Bárbara Della Noce²  Camila Fernanda Waltero²  Evenilton Pessoa Costa²  Leonardo Araujo de Abreu²  Naftaly Wang’ombe Githaka¹  Jorge Moraes³  Helga Fernandes Gomes³  Satoru Konnai¹  Itabajara da Silva Vaz⁴  Kazuhiko Ohashi¹  Carlos Logullo² 

[1] Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Kita-ku Sapporo 060-0818, Japan; E-Mails:;Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil; E-Mails:;Laboratory of Biochemistry Hatisaburo Masuda, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, NUPEM-UFRJ/Macaé, Campus Macaé, Avenida São José do Barreto, São José do Barreto, CEP 27965-045 Macaé, RJ, Brazil; E-Mails:;Center of Biotechnology, Federal University of Rio Grande do Sul, C.P. 15005, Av. Bento Gonçalves 9500, Prédio 43421, Campos do Vale, CEP 91501-970 Porto Alegre, RS, Brazil; E-Mail:

关键词: metabolism;    gluconeogenesis;    glycolysis;    tick;    gene expression;    glucose;   

DOI  :  10.3390/ijms16011821

来源: mdpi

【 摘 要 】

In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with these pathways are modulated by alterations in energy resource availability (primarily glucose). BME26 cells in media were investigated using three different glucose concentrations, and changes in the transcription levels of target genes in response to carbohydrate utilization were assessed. The results indicate that several genes, such as glycogen synthase (GS), glycogen synthase kinase 3 (GSK3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6 phosphatase (GP) displayed mutual regulation in response to glucose treatment. Surprisingly, the transcription of gluconeogenic enzymes was found to increase alongside that of glycolytic enzymes, especially pyruvate kinase, with high glucose treatment. In addition, RNAi data from this study revealed that the transcription of gluconeogenic genes in BME26 cells is controlled by GSK-3. Collectively, these results improve our understanding of how glucose metabolism is regulated at the genetic level in tick cells.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.