Renin-angiotensin system inhibitor attenuates oxidative stress induced human coronary artery endothelial cell dysfunction via the PI3K/AKT/mTOR pathway

Archives of Medical Science

卷:15

关键词: apoptosis;    oxidative stress;    renin-angiotensin system inhibitor;    akt/mtor pathway;    endothelial cell dysfunction;    renin-angiotensin system inhibitor;    oxidative stress;    apoptosis;    endothelial cell dysfunction;   

DOI  :  10.5114/aoms.2018.74026

来源: DOAJ

【 摘 要 】

IntroductionThe renin-angiotensin system is associated with blood pressure regulation, inflammation, oxidative stress and insulin resistance. It can decrease intracellular oxidative stress. Stimulation with H 2 O 2leads to increased oxidative stress and activation of the AKT/mTOR pathway. However, the role of renin-angiotensin system inhibitors in oxidative stress-induced endothelial cell dysfunction and H 2 O 2 -induced AKT activation remains unclear.Material and methodsHuman coronary artery endothelial cells (HCAECs) were used. The cells were treated with H 2 O 2 , captopril, the AKT inhibitor MK-2206, and the AKT activator SC79, either separately, or in combination. p53 and ICAM-1 expression, and p-eNOS, p-Akt and mTOR activation were measured by Western blot. Cell viability was assessed by MTT assay. Levels of reactive oxygen species (ROS) were assayed by flow cytometry. Proliferation was monitored by BrdU labeling, while cell migration and invasion were determined by wound healing and Transwell assays, respectively.ResultsThe renin-angiotensin system inhibitor captopril reversed H 2 O 2 -induced oxidative stress and apoptosis in HCAECs. Co-treatment with captopril and the AKT inhibitor MK-2206 reduced the H 2 O 2 -induced P53 and ICAM-1 protein expression (p < 0.05). The proliferation, migration and invasion of HCAECs were significantly enhanced by co-treatment with captopril and MK-2206 (p < 0.05).ConclusionsThe study revealed the protective effect of captopril against H 2 O 2 -induced endothelial cell dysfunction through the AKT/mTOR pathway, and its enhancement of cell survival. These findings provide new insights into the protective effects of captopril and novel therapeutic approaches to treatment of cardiovascular disease.

【 授权许可】

Unknown