2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of β-carotene, zeaxanthin, and ketocarotenoids in rice endosperm

Metabolic Engineering Communications

卷:12

Hyung-Keun Ku¹  Young-Joo Jung²  Dongho Lee³  Ye Sol Jeong⁴  Jae Kwang Kim⁴  Kyoung Bok Lee⁴  Ju-Kon Kim⁵  Sun-Hyung Lim⁵  Sun-Hwa Ha⁶ 

[1] Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea;

[2] National Academy of Agricultural Science, Rural Development Administration, Jeonju, 54874, Republic of Korea;

[3] Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, 25354, Republic of Korea;

[4] Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, 17104, Republic of Korea;

[5] Division of Life Sciences and Bio-Resource and Environmental Center, Incheon National University, Incheon, 22012, Republic of Korea;

关键词: 2A peptide;    Carotenoid;    Metabolic engineering;    Polycistron;    Rice;   

DOI  :  

来源: DOAJ

【 摘 要 】

Foot-and-mouth disease virus (FMDV) 2A constructs have been successfully used for the production of “Golden Rice”, a β-carotene producing rice strain. However, to allay public fears and opposition to plants carrying a mammalian pathogenic viral sequence, 2A-like synthetic sequences from Thosea asigna virus and Infectious myonecrosis virus were used to coordinate the coexpression of carotenoid biosynthetic genes. Here, up to four carotenogenic genes encoding PSY, CRTI, BCH and BKT were concatenated and produced β-carotene, zeaxanthin, and ketocarotenoids (astaxanthin and adonixanthin) in transgenic rice seeds displaying color variation due to the difference in carotenoid content and composition.

【 授权许可】

Unknown