2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology

Viruses

Daniel C. F. Lanza¹  Juliana G. S. de Lima¹ 

[1] Applied Molecular Biology Lab—LAPLIC, Department of Biochemistry, Federal University of Rio Grande do Norte, Natal 59064-720, Brazil;

关键词: 2A peptide;    double-stranded RNA virus;    positive-sense single-stranded RNA virus;    Totiviridae;    Picornaviridae;   

DOI  :  10.3390/v13112160

来源: DOAJ

【 摘 要 】

2A is an oligopeptide sequence that mediates a ribosome “skipping” effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed.

【 授权许可】

Unknown