Hydrogel composite scaffolds achieve recruitment and chondrogenesis in cartilage tissue engineering applications

Journal of Nanobiotechnology

Mingxue Chen¹  Liping Wu²  Shuangpeng Jiang³  Zhen Yang³  Kangkang Zha³  Pinxue Li³  Yu Yang³  Xujiang Luo³  Guangzhao Tian³  Xiang Sui³  Shuyun Liu³  Quanyi Guo³  Liqing Peng⁴  Bo Huang⁴  Huo Li⁴  Hao Wang⁴  Qinyu Tian⁴ 

[1] Department of Orthopaedics, Beijing Jishuitan Hospital, 100035, Beijing, China;Hebei Medical University, 050017, Shijiazhuang, Hebei, China;Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No.28 Fuxing Road, Haidian District, 100853, Beijing, People’s Republic of China;Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Lab of Regenerative Medicine in Orthopedics, Key Laboratory of Musculoskeletal Trauma & War Injuries PLA, No.28 Fuxing Road, Haidian District, 100853, Beijing, People’s Republic of China;Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, No. 25 Taiping Road, Jiangyang District, 646000, Luzhou, Sichuan, People’s Republic of China;

关键词: Decellularized extracellular matrix;    Peptide;    Recruitment;    Chondrogenesis;    Cartilage regeneration;   

DOI  :  10.1186/s12951-021-01230-7

来源: Springer

【 摘 要 】

BackgroundThe regeneration and repair of articular cartilage remains a major challenge for clinicians and scientists due to the poor intrinsic healing of this tissue. Since cartilage injuries are often clinically irregular, tissue-engineered scaffolds that can be easily molded to fill cartilage defects of any shape that fit tightly into the host cartilage are needed.MethodIn this study, bone marrow mesenchymal stem cell (BMSC) affinity peptide sequence PFSSTKT (PFS)-modified chondrocyte extracellular matrix (ECM) particles combined with GelMA hydrogel were constructed.ResultsIn vitro experiments showed that the pore size and porosity of the solid-supported composite scaffolds were appropriate and that the scaffolds provided a three-dimensional microenvironment supporting cell adhesion, proliferation and chondrogenic differentiation. In vitro experiments also showed that GelMA/ECM-PFS could regulate the migration of rabbit BMSCs. Two weeks after implantation in vivo, the GelMA/ECM-PFS functional scaffold system promoted the recruitment of endogenous mesenchymal stem cells from the defect site. GelMA/ECM-PFS achieved successful hyaline cartilage repair in rabbits in vivo, while the control treatment mostly resulted in fibrous tissue repair.ConclusionThis combination of endogenous cell recruitment and chondrogenesis is an ideal strategy for repairing irregular cartilage defects.Graphical Abstract

【 授权许可】

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