Image-guided selection of Gd@C-dots as sensitizers to improve radiotherapy of non-small cell lung cancer

Journal of Nanobiotechnology

Fangchao Jiang¹  Chaebin Lee¹  Jin Xie¹  Guihua Jiang²  Xiaofen Ma³  Tao Zhang⁴  Zibo Li⁴  Hui Wang⁴  Jinghua Cai⁴  Zhanhong Wu⁴ 

[1] Department of Chemistry, University of Georgia, 140 Cedar Street, 30602, Athens, GA, USA;Department of Nuclear Medicine, Guangdong Second Provincial General Hospital, 466 Xingang Middle Road, Haizhu District, 510317, Guangzhou City, Guangdong Province, People’s Republic of China;Department of Nuclear Medicine, Guangdong Second Provincial General Hospital, 466 Xingang Middle Road, Haizhu District, 510317, Guangzhou City, Guangdong Province, People’s Republic of China;Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, 27599, Chapel Hill, NC, USA;Department of Radiology, Biomedical Research Imaging Center, and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 125 Mason Farm Road, 27599, Chapel Hill, NC, USA;

关键词: Gadolinium;    Carbon dots;    Radiosensitizers;    PET imaging;    Non-small cell lung cancer;    Radiation therapy;   

DOI  :  10.1186/s12951-021-01018-9

来源: Springer

【 摘 要 】

BackgroundRecently, gadolinium-intercalated carbon dots (Gd@C-dots) have demonstrated potential advantages over traditional high-Z nanoparticles (HZNPs) as radiosensitizers due to their high stability, minimal metal leakage, and remarkable efficacy.ResultsIn this work, two Gd@C-dots formulations were fabricated which bore carboxylic acid (CA-Gd@C-dots) or amino group (pPD-Gd@C-dots), respectively, on the carbon shell. While it is critical to develop innovative nanomateirals for cancer therapy, determining their tumor accumulation and retention is equally important. Therefore, in vivo positron emission tomography (PET) was performed, which found that 64Cu-labeled pPD-Gd@C-dots demonstrated significantly improved tumor retention (up to 48 h post injection) compared with CA-Gd@C-dots. Indeed, cell uptake of 64Cu-pPD-Gd@C-dots reached close to 60% of total dose compared with ~ 5% of 64Cu-CA-Gd@C-dots. pPD-Gd@C-dots was therefore further evaluated as a new radiosensitizer for non-small cell lung cancer treatment. While single dose radiation plus intratumorally injected pPD-Gd@C-dots did lead to improved tumor suppression, the inhibition effect was further improved with two doses of radiation. The persistent retention of pPD-Gd@C-dots in tumor region eliminates the need of reinjecting radiosensitizer for the second radiation.ConclusionsPET offers a simple and straightforward way to study nanoparticle retention in vivo, and the selected pPD-Gd@C-dots hold great potential as an effective radiosensitizer.Graphic abstract

【 授权许可】

CC BY