Molecular detection, phylogenetic analysis and genetic diversity of recently isolated foot-and-mouth diseasevirus serotype A African topotype, Genotype IV

Virology Journal

Mostafa R. Zaher¹  Naglaa M. Hagag¹  Ayah M. Hassan¹  Essam M. Ibraheem²  Mervat I. Abd-El-Moniem³  Rabab T. Hassanien³  Ahmed R. Habashi³  Momtaz A. Shahein³  Mohamed E. El Zowalaty⁴ 

[1] Genome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), 12618, Dokki, Giza, Egypt;Pathology Research Department, Animal Health Research Institute, Agriculture Research Center (ARC), 12618, Dokki, Giza, Egypt;Virology Research Department, Animal Health Research Institute, Agriculture Research Center (ARC), 12618, Dokki, Giza, Egypt;Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, SE-75 123, Uppsala, Sweden;

关键词: Foot and mouth disease virus;    FMDV;    Epidemiology;    Variant;    Genotype IV;    Phylogenetic analysis;    VP1 gene;   

DOI  :  10.1186/s12985-021-01693-y

来源: Springer

【 摘 要 】

BackgroundSurveillance for circulating emerging diseases of economic importance has a major role in the rapid response to major pathogen outbreaks. Foot-and-mouth disease virus (FMDV) is one of the significant endemic viruses in Egypt. FMDV is periodically investigated for monitoring evolution and emergence of new variants. The genetic characterization of foot-and-mouth disease (FMD) virus serotype A responsible for recent outbreaks of FMD in Egypt was determined.MethodsSamples were collected from different locations and virus isolation was performed using BHK-21 cells. Viral RNA was extracted and samples were screened for FMDV using real-time RT-PCR. DNA sequence analysis was performed and computational and bioinformatics analyses were used to determine the substitution rates and phylogenetic relationship.ResultsSequence and phylogenetic analyses of full-length 1D region of FMDV samples collected from different governorates in 2020 showed close similarity to Egyptian FMDV strains from serotype A-African topotype-G-IV with genetic variation of 6.5%. Recently isolated FMDV strains showed high genetic variations from locally used vaccine strains in the major antigenic sites of VP1 region.ConclusionsAlthough, efforts made by the veterinary authorities to implement an effective mass vaccination plan, the recently detected FMDV strains in this study could not be subtyped using the FMDV primers routinely used for molecular serotyping. These dissimilarities raise the alarm for reconsideration of the FMDV isolates used in vaccine manufacture. Clearly close monitoring of FMD in Egypt is urgently required to define the risks of future outbreaks and to ensure appropriate control measures against FMD major outbreaks.

【 授权许可】

CC BY