Low-cost production and application of lipopeptide for bioremediation and plant growth by Bacillus subtilis SNW3

AMB Express

Safia Ahmed¹  Afshan Hina Naeem¹  Aneeqa Zafar¹  Aiman Umar¹  Muneer Ahmed Qazi²  Hasina Wali³  Zulfiqar Ali Malik⁴  Meh Para Siddique⁵ 

[1] Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan;Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan;Department of Microbiology, Shah Abdul Latif University, 66111, Khairpur, Sindh, Pakistan;Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, 45320, Islamabad, Pakistan;Department of Microbiology, University of Balochistan, 87300, Quetta, Pakistan;Department of Microbiology, Shah Abdul Latif University, 66111, Khairpur, Sindh, Pakistan;Department of Psychology, Quaid-i-Azam University, 45320, Islamabad, Pakistan;

关键词: Ecotoxicity;    Bacillus subtilis;    Lipopeptide;    Bioremediation;    Plant growth promotion;   

DOI  :  10.1186/s13568-021-01327-0

来源: Springer

【 摘 要 】

At present time, every nation is absolutely concern about increasing agricultural production and bioremediation of petroleum-contaminated soil. Hence, with this intention in the current study potent natural surfactants characterized as lipopeptides were evaluated for low-cost production by Bacillus subtilis SNW3, previously isolated from the Fimkessar oil field, Chakwal Pakistan. The significant results were obtained by using substrates in combination (white beans powder (6% w/v) + waste frying oil (1.5% w/v) and (0.1% w/v) urea) with lipopeptides yield of about 1.17 g/L contributing 99% reduction in cost required for medium preparation. To the best of our knowledge, no single report is presently describing lipopeptide production by Bacillus subtilis using white beans powder as a culture medium. Additionally, produced lipopeptides display great physicochemical properties of surface tension reduction value (SFT = 28.8 mN/m), significant oil displacement activity (ODA = 4.9 cm), excessive emulsification ability (E24 = 69.8%), and attains critical micelle concentration (CMC) value at 0.58 mg/mL. Furthermore, biosurfactants produced exhibit excellent stability over an extensive range of pH (1–11), salinity (1–8%), temperature (20–121°C), and even after autoclaving. Subsequently, produced lipopeptides are proved suitable for bioremediation of crude oil (86%) and as potent plant growth-promoting agent that significantly (P < 0.05) increase seed germination and plant growth promotion of chili pepper, lettuce, tomato, and pea maximum at a concentration of (0.7 g/100 mL), showed as a potential agent for agriculture and bioremediation processes by lowering economic and environmental stress.Graphical Abstract

【 授权许可】

CC BY