The effect of lipocalin-2 (LCN2) on apoptosis: a proteomics analysis study in an LCN2 deficient mouse model

BMC Genomics

Ye Han¹  Dongming Wu¹  Yayun Wang¹  Xiaopeng Wang¹ 

[1] Department of Emergency, Qingdao Hospital of Traditional Chinese Medicine (Qingdao Hiser Hospital), No. 4, Renmin Road, Shibei District, 266033, Qingdao, Shandong Province, China;

关键词: Lipocalin 2;    Label-free proteomics;    Apoptosis;   

DOI  :  10.1186/s12864-021-08211-y

来源: Springer

【 摘 要 】

BackgroundRecent studies have shown that lipocalin-2 (LCN2) has multiple functions involved in various biological and pathological processes including energy homeostasis, cancer, inflammation, and apoptosis. We aimed to investigate the effect of LCN2 on apoptosis that influences the pathogenetic process of metabolic diseases and cancer.MethodsWe performed a proteomics analysis of livers taken from LCN2-knockout mice and wild type mice by using label-free LC-MS/MS quantitative proteomics.ResultsProteomic analysis revealed that there were 132 significantly differentially expressed proteins (49 upregulated and 83 downregulated) among 2140 proteins in the liver of LCN2-knockout mice compared with wild type mice. Of these, seven apoptosis-associated proteins were significantly upregulated and seven apoptosis-associated proteins downregulated.ConclusionProteomics demonstrated that there were seven upregulated and seven downregulated apoptosis-associated proteins in liver of LCN2-knockout mice. It is important to clarify the effect of LCN2 on apoptosis that might contribute to the pathogenesis of insulin resistance, cancer, and various nervous system diseases.

【 授权许可】

CC BY