Clinical implications and molecular mechanisms of Cyclin-dependent kinases 4 for patients with hepatocellular carcinoma

BMC Gastroenterology

Xi-wen Liao¹  Tao Peng¹  Chen-lu Lan¹  Zhong-liu Wei¹  Xin Zhou¹  Shu-tian Mo¹  Hua-sheng Huang¹  Yong-guang Wei¹ 

[1] Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, 530021, Nanning, Guangxi Zhuang Autonomous Region, People’s Republic of China;

关键词: Cyclin-dependent kinases;    Hepatocellular carcinoma;    Prognosis;    Biomarker;   

DOI  :  10.1186/s12876-022-02152-w

来源: Springer

【 摘 要 】

BackgroundHepatocellular carcinoma(HCC) was frequently considered as a kind of malignant tumor with a poor prognosis. Cyclin-dependent kinases(CDK) 4 was considered to be cell-cycle-related CDK gene. In this study, we explored the clinical significance of CDK4 in HCC patients.MethodsData of HCC patients were obtained from The Cancer Genome Atlas database(TCGA) and the Gene Expression Omnibus(GEO) database. Kaplan–Meier analysis and Cox regression model were performed to calculate median survival time(MST) and the hazard ration(HR), respectively. The joint-effect analysis and prognostic risk score model were constructed to demonstrate significance of prognosis-related genes. The differential expression of prognostic genes was further validated using reverse transcription-quantitative PCR(RT-qPCR) of 58 pairs of HCC samples.ResultsCDK1 and CDK4 were considered prognostic genes in TCGA and GSE14520 cohort. The result of joint-effect model indicated patients in CDK1 and CDK4 low expression groups had a better prognosis in TCGA(adjusted HR = 0.491; adjusted P = 0.003) and GSE14520 cohort(adjusted HR = 0.431; adjusted P = 0.002). Regarding Kaplan–Meier analysis, high expression of CDK1 and CDK4 was related to poor prognosis in both the TCGA(P < 0.001 and = 0.001 for CDK1 and CDK4, respectively) and the GSE14520 cohort(P = 0.006 and = 0.033 for CDK1 and CDK4, respectively). However, only CDK4(P = 0.042) was validated in RT-qPCR experiment, while CDK1(P = 0.075) was not.ConclusionHCC patients with high CDK4 expression have poor prognosis, and CDK4 could be a potential candidate diagnostic biomarker for HCC.

【 授权许可】

CC BY