Origanum vulgare L. essential oil inhibits virulence patterns of Candida spp. and potentiates the effects of fluconazole and nystatin in vitro

BMC Complementary Medicine and Therapies

Rocío Santander¹  Raúl Quijada²  Andrea Müller-Sepúlveda³  Rodrigo López-Muñoz⁴  Camila Cid-Chevecich⁵  Alejandro Escobar⁵  Mario Díaz-Dosque⁵  Mauricio Budini⁵  José Antonio Jara⁵  Alfredo Molina-Berríos⁵  Alfredo Criollo⁵ 

[1] Department of Environmental Sciences, Faculty of Chemistry and Biology, Universidad de Santiago de Chile, Santiago, Chile;Faculty of Physical and Mathematical Sciences, Universidad de Chile, Santiago, Chile;Institute of Agrifood, Animals and Environmental Sciences, Universidad de O’Higgins, San Fernando, Chile;Instituto de Farmacología y Morfofisiología, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile;Laboratory of Pharmacology, Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Olivos 943, Independencia, Santiago, Chile;

关键词: Candida albicans;    Essential oils;    Biofilms;    Origanum vulgare;   

DOI  :  10.1186/s12906-022-03518-z

来源: Springer

【 摘 要 】

BackgroundRecurrence and resistance of Candida spp. infections is associated with the ability of these microorganisms to present several virulence patterns such as morphogenesis, adhesion, and biofilm formation. In the search for agents with antivirulence activity, essential oils could represent a strategy to act against biofilms and to potentiate antifungal drugs.ObjectiveTo evaluate the antivirulence effect of Origanum vulgare L. essential oil (O-EO) against Candida spp. and to potentiate the effect of fluconazole and nystatin.MethodsThe effect of O-EO was evaluated on ATCC reference strains of C. albicans and non-albicans Candida species. Minimum inhibitory concentration (MIC) was determined through broth microdilution assay. Adhesion to microplates was determined by crystal violet (CV) assay. An adapted scratch assay in 24-well was used to determine the effect of essential oil on biofilms proliferation. Viability of biofilms was evaluated by MTT reduction assay and through a checkerboard assay we determined if O-EO could act synergistically with fluconazole and nystatin.ResultsMIC for C. albicans ATCC-90029 and ATCC-10231 was 0.01 mg/L and 0.97 mg/L, respectively. For non-albicans Candida strains MIC values were 2.6 mg/L for C. dubliniensis ATCC-CD36 and 5.3 mg/L for C. krusei ATCC-6258. By using these concentrations, O-EO inhibited morphogenesis, adhesion, and proliferation at least by 50% for the strains assayed. In formed biofilms O-EO decreased viability in ATCC 90029 and ATCC 10231 strains (IC50 7.4 and 2.8 mg/L respectively). Finally, we show that O-EO interacted synergistically with fluconazole and nystatin.ConclusionsThis study demonstrate that O-EO could be considered to improve the antifungal treatment against Candida spp.

【 授权许可】

CC BY