Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris

Chemistry Central Journal

Diana Obistioiu¹  Romeo T Cristina¹  Ivo Schmerold⁴  Remigius Chizzola²  Klaus Stolze⁴  Ileana Nichita¹  Viorica Chiurciu³ 

[1] Banat’s University of Agricultural Sciences and Veterinary Medicine from Timisoara, Faculty of Veterinary Medicine, Calea Aradului no 119, Timisoara 300645, Romania

[2] Institute for Applied Botany and Pharmacognosy, University of Veterinary Medicine Vienna, Vienna A - 1210, Austria

[3] Romvac, Voluntari, Bucuresti 061182, Romania

[4] Department for Biomedical Sciences, Institute of Pharmacology and Toxicology, University of Veterinary Medicine Vienna, Vienna A-1210, Austria

关键词: Candida albicans;    Antifungal activity;    GC-MS;    Essential oils;    Artemisia;   

Others  :  787805
DOI  :  10.1186/1752-153X-8-6

 received in 2013-09-13, accepted in 2014-01-27,  发布年份 2014

【 摘 要 】

Background

A large number of essential oils is reported to have significant activity against Candida albicans. But the different chemical composition influences the degree of their activity. The intention of this study was to investigate the chemical composition and the activity against Candida albicans of volatile oils obtained from Artemisia dracunculus, A. abrotanum, A. absinthium and A. vulgaris (Asteraceae). The aim of the study was to identify new chemical compounds that have effect against C. albicans.

The essential oils were obtained by hydrodistillation or extraction with dichloromethane (a new procedure we developed trying to obtain better, more separated compounds) from air dried above ground plant material and analyzed by GC-MS. Additionally commercial essential oils from the same species were tested. The Candida albicans inhibition studies were carried out by the paper disc diffusion method.

Results

The essential oils shared common components but presented differences in composition and showed variable antifungal activity. Davanone and derivatives thereof, compounds with silphiperfolane skeleton, estragole, davanone oil, β-thujone, sabinyl acetate, herniarin, cis-chrysanthenyl acetate, 1,8-cineol, and terpineol were the main components of Artemisia volatiles.

Conclusions

Among the volatile fractions tested those from A. abrotanum containing davanone or silphiperfolane derivatives showed the highest antifungal activity. The in vitro tests revealed that the Artemisia oils are promising candidates for further research to develop novel anti-candida drugs.

【 授权许可】

   
2014 Obistioiu et al.; licensee Chemistry Central Ltd.

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